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a(chn)Ʒ

ACAT2,oøAD(zhun)ø2ELISAԇжX

ACAT2,oøAD(zhun)ø2ELISAԇжX

rh

ُȣ159    ԭa(chn)أ

l(f)rg2018/7/25 9:23:34

ACAT2,oøAD(zhun)ø2ELISAԇжXwⶨzyѪѪ{M弰P(gun)ҺwӱдoøAD(zhun)ø2ACAT2ĺ

(bio)ACAT2   oøAD(zhun)ø2   ELISAԇжX   

ԔϢ
a(chn)Ʒ
a(chn)ƷQ
ACAT2,oøAD(zhun)ø2elisaԇX
λУ/t(y)Ժ/Ժ
ͣAķ/gӷ/
(yng)rg: 1-5h 
ӱwe: 50-100ul 
zyL: 450 nm
(yng)÷wⶨzyѪѪ{M弰P(gun)Һwӱ
l2-8
•a(chn)ƷHЌ򞣬mR\
a(chn)Ʒ
΢יzyϵУ׽ԇ,TNFԇ,VEGFԇ,Ipԇ
P(gun)СәzyϵУƤ|(zh)ԇ,HCYԇ,T3,T4ԇУ
ʳƷȫСәzyϵУcùELISAzyԇ,谷ELISAzyԇ,ĭh(hun)ELISAzyԇ,SùELISAzyԇ
wzyϵУѪowELISAzyԇ,AQP-Abԇ,HINIԇУ
 
طNיzyʮNٵİ׵,򵰰ELISAzyԇ
 
 My
ֻјӱ^҂?yu)?ji)ʡrgY(ji)ԭʼ(sh)(j)(sh)(j)ṩrg5-7ң


g(sh)ָ(do)
ȫ̼g(sh)ָ(do)ǰĘ(bio)ռʹ^в׵ĵطY(ji)Ĕ(sh)(j)ȵ߅elisaԇЌ򞌣

λ
ACAT2,oøAD(zhun)ø2ELISAԇжX͑Ⱥw

ӆُ
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gӭĿІλڸNĿc˾_չͬӴεкpAl(f)չͬMЇzy˜I(y)İl(f)չe۽(jng)򞡣

 
(bio)Ҫ
1. Ѫ壺ȫѪ(bio)ՈҜط2Сr4^ҹ1000gx20ȡ弴əzy?q)?bio)-20-80汣(yng)ⷴ(f)ڡ
2. Ѫ{EDTA鿹(bio)ɼ30犃(ni)2 - 8°C 1000gx20?q)?bio)-20-80汣棬(yng)ⷴ(f)
3. M{A(y)PBS (0.01M, pH=7.4)_ϴMȥѪҺ{ѽļtӰ표yY(ji)Qغ󌢽M顣ĽMc(yng)wePBSһ㰴1:9we1gĽMƷ(yng)9mLPBSwweɸ(j)Ҫm(dng){(dio)ӛ]PBSм뵰øƄ벣{УڱϳĥMһѽMԌ{ҺMг•飬򷴏(f)*󌢄{Һ5000×gx5~10犣ȡzy
4. B(yng)(bio)1000gx20ȡ弴əzy?q)?bio)-20-80汣(yng)ⷴ(f)
ע(bio)ѪӰ*zyY(ji)Ѫ(bio)Mд헙zy

ACAT2,oøAD(zhun)ø2ELISAԇжX(bio)̎
1. ˾ֻԇбؓ؟(z)ʹԓԇɵĘӱؓ؟(z)Ոʹʹǰֿ]ӱĿʹA(y)Ęӱ
2. ǰ(yng)A(y)y(bio)(bio)^(yng)(bio)MϡጣʹϡጺĘ(bio)ԇеęzyӋrٳ(yng)ϡጱ(sh)(bio)ʹ0.01mol/LPBSϡ(PH=7.0-7.2)
3. zӱfИӱ֮hMA(y)CЧԣעӱ
4. ʹûW(xu)ѽҺƂĽM{򼚰ȡҺܕijЩW(xu)|(zh)댧(do)ELISAY(ji)ƫ
5. ӱ鼚B(yng)ԓӱɔ_^磺B(ti)(sh)ɘӕrgԿܴڙzyr
6. ijЩȻ׻ؽMԭ˼ؽMca(chn)Ʒʹõęzyw@wƥzy
7. hʹrӱrg^Lܕڵ׽׃Ԍ(do)ŒY(ji)ƫ

 

•rg2024/11/18 9:49:29

ھ

ھԃ𾴵Ŀ͑҂M؏(f)ԃ

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xW(wng) O(sh)Ӌδ(jng)S䛱ؾ. “(lin)ϵˣX(jng)“(lin)ϵԒ13636351217 ICP䰸̖ICP11004148̖-11 L8851481

Ia(chn)ƷELISAԇСELISAԇELISAԇССELISAԇԇwѪMژ(bio)(zhn)Ʒ΢B(yng)

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