λУ/t(y)Ժ/Ժ
ͣAķ/gӷ/
(yng)rg: 1-5h
ӱwe: 50-100ul
zyL: 450 nm
(yng)÷wⶨzyѪѪ{M弰P(gun)Һwӱ
l2-8
a(chn)ƷHЌmR\
a(chn)Ʒ
יzyϵУԇ,TNFԇ,VEGFԇ,Ipԇ
P(gun)СәzyϵУƤ|(zh)ԇ,HCYԇ,T3,T4ԇУ
ʳƷȫСәzyϵУcùELISAzyԇ,谷ELISAzyԇ,ĭh(hun)ELISAzyԇ,SùELISAzyԇ
wzyϵУѪowELISAzyԇ,AQP-Abԇ,HINIԇУ
طNיzyʮNٵİ,ELISAzyԇ
My
ֻјӱ^҂?yu)?ji)ʡrgY(ji)ԭʼ(sh)(j)(sh)(j)ṩrg5-7ң
g(sh)ָ(do)
ȫ̼g(sh)ָ(do)ǰĘ(bio)ռʹ^вĵطY(ji)Ĕ(sh)(j)ȵ߅elisaԇЌ
λACAT2,oøAD(zhun)ø2ELISAԇжX͑Ⱥwӆُ
ӆُ̣
gӭĿІλڸNĿc˾_չͬӴεкpAl(f)չͬMЇzyI(y)İl(f)չe۽(jng)
(bio)Ҫ1. Ѫ壺ȫѪ(bio)ՈҜط2Сr4^ҹ1000gx20ȡ弴əzy?q)?bio)-20-80汣(yng)ⷴ(f)ڡ2. Ѫ{EDTA鿹(bio)ɼ30犃(ni)2 - 8°C 1000gx20?q)?bio)-20-80汣棬(yng)ⷴ(f)3. M{A(y)PBS (0.01M, pH=7.4)_ϴMȥѪҺ{ѽļtӰ표yY(ji)QغM顣ĽMc(yng)wePBSһ㰴1:9we1gĽMƷ(yng)9mLPBSwweɸ(j)Ҫm(dng){(dio)ӛ]PBSм뵰øƄ벣{УڱϳĥMһѽMԌ{ҺMг飬(f)*{Һ5000×gx5~10犣ȡzy4. B(yng)(bio)1000gx20ȡ弴əzy?q)?bio)-20-80汣(yng)ⷴ(f)ע(bio)ѪӰ*zyY(ji)Ѫ(bio)Mд헙zyACAT2,oøAD(zhun)ø2ELISAԇжX(bio)̎1. ˾ֻԇбؓ؟(z)ʹԓԇɵĘӱؓ؟(z)Ոʹʹǰֿ]ӱĿʹA(y)Ęӱ2. ǰ(yng)A(y)y(bio)(bio)^(yng)(bio)MϡጣʹϡጺĘ(bio)ԇеęzyӋrٳ(yng)ϡጱ(sh)(bio)ʹ0.01mol/LPBSϡ(PH=7.0-7.2)3. zӱfИӱ֮hMA(y)CЧԣעӱ4. ʹûW(xu)ѽҺƂĽM{ȡҺܕijЩW(xu)|(zh)댧(do)ELISAY(ji)ƫ5. ӱ鼚B(yng)ԓӱɔ_^磺B(ti)(sh)ɘӕrgԿܴڙzyr6. ijЩȻؽMԭ˼ؽMca(chn)Ʒʹõęzyw@wƥzy7. hʹrӱrg^Lܕڵ׃Ԍ(do)Y(ji)ƫ
rg2024/11/18 9:49:29