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ELISAԇСELISAԇСELISAԇСELISAԇСԇwѪM(jn)ژ(bio)(zhn)Ʒ΢B(yng)
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a(chn)Ʒ

BIMţY(ji)˲TBELISAԇ

BIMţY(ji)˲TBELISAԇ

r(ji)h

ُȣ155    ԭa(chn)أ

l(f)r(sh)g2018/8/13 15:52:14

BIMţY(ji)˲TBELISAԇwⶨzyѪѪ{M(x)弰P(gun)ҺwӱţY(ji)˲TBĺ

Ԕ(x)Ϣ
a(chn)ƷQBIMţY(ji)˲TBelisaԇ
λУ/t(y)Ժ/Ժ
(sh)(yn)ͣAķ/gӷ/
(yng)r(sh)g: 1-5h
ӱwe: 50-100ul
zyL: 450 nm
(yng)÷wⶨzyѪѪ{M(x)弰P(gun)Һwӱ
l2-8棻
•a(chn)ƷHЌ(sh)(yn)mR\

a(chn)Ʒ
΢יzyϵУ׽ԇ,TNFԇ,VEGFԇ,IpԇУ
P(gun)СәzyϵУƤ|(zh)ԇ,HCYԇ,T3,T4ԇ
ʳƷȫСәzyϵУcùELISAzyԇ,谷ELISAzyԇ,ĭh(hun)ELISAzyԇ,SùELISAzyԇУ
wzyϵУѪowELISAzyԇ,AQP-Abԇ,HINIԇ
طNיzyʮ(g)Nٵİ׵,򵰰ELISAzyԇС
M(fi)y
ֻјӱ^҂?yu)?ji)ʡr(sh)gY(ji)ԭʼ(sh)(j)(sh)(j)ṩr(sh)g5-7ң


g(sh)ָ(do)
ȫ̼g(sh)ָ(do)ǰĘ(bio)ռʹ^в׵ĵط(sh)(yn)Y(ji)Ĕ(sh)(j)ȵ߅elisaԇ(sh)(yn)ҡ

BIMţY(ji)˲TBELISAԇ͑Ⱥw

ӆُ̣

gӭĿІλڸN(xing)Ŀc˾_չͬӴεкpAl(f)չͬM(jn)Їzy˜I(y)İl(f)չe۽(jng)(yn)

E
1. Ҝƽ20minXȡlʣlԷܷŻ4
2. O(sh)Ø(bio)(zhn)Ʒ׺͘ӱ(bio)(zhn)Ʒ׸ӲͬȵĘ(bio)(zhn)Ʒ50μL
3. ӱмyӱ50μLհ׿ײ
4. հ׿(bio)(zhn)Ʒ׺͘ӱÿ׼^øHRP(bio)ӛęzyw100μL÷Ĥס(yng)37ˮԡ偻60min
5. ȥҺwˮĸÿ׼ӝMϴҺ350μLo1min˦ȥϴҺˮĸ؏(f)ϴ5ΣҲϴC(j)ϴ壩
6. ÿ׼AB50μL37ܹ15min
ÿ׼KֹҺ50μL15min(ni)450nmL̎y׵ODֵ

BIMţY(ji)˲TBELISAԇ
ע(xing)
1.(yn)Ҏ(gu)ĕr(sh)g͜ضM(jn)МԱC(zhn)_Y(ji)ԇʹǰ_(d)Ҝ20-25ʹúرԇ
2.ϴ岻_Ԍ(do)²(zhn)_ĽY(ji)ڼǰ_Mɿ׃(ni)Һw^вҪ׌΢׸
3.ךҺwָӡtӰODֵ
4.@ɫҺ(yng)ʟoɫܜ\ɫѽ(jng)׃{(ln)ĵҺʹ
5.ԇ͘(bio)ĽȾe(cu)`Y(ji)
6.ڃ(ch)͜r(sh)⏊(qing)ֱ䡣
7.ƽҜغٴ_ܷԷˮl
8.κη(yng)ԇܽ|Ư܄Ư܄ɢl(f)ď(qing)ҚwκƯ׳ɷֶ(hu)Ɖԇз(yng)ԇԡ
9.ʹ^ڮa(chn)Ʒ
܂еĘƷ(yng)Ҏ(gu)ij̎Ʒ͙zyb

•r(sh)g2024/11/18 9:49:47

ھ

ھԃ𾴵Ŀ͑҂(hu)M؏(f)ԃ

dȤĮa(chn)Ʒ* ߀]ݔdȤĮa(chn)Ʒ
Ćλ* ߀]ݔĆλ
“(lin)ϵ* ߀]ݔ“(lin)ϵ
“(lin)ϵԒ* ߀]ݔ“(lin)ϵԒ
Ԕ(x)ַ
] ݔ]ʽ_
Ո(qng)ݔ(du)҂Ҋh* ߀]ݔ(du)҂Ҋh
(yn)Ca* ߀]ݔ(yn)Ca

l(f)

ܰʾ

1.A񹲺͇P(gun)Ҏ(gu)ؾW(wng)ϵ£Г(dn)һОֱӻgķ؟(z)

2.Ո(qng)挍(sh)ķӳa(chn)Ʒr,Ҫ졢_{猦(du)a(chn)ƷκɆ,Ҳԃ

3.δ(jng)վͬκ˲ñԲl(f)(g)˻F(tun)wľЏV|(zh)ϢՓ

P(gun)

| ˾B| a(chn)Ʒչʾ| ˾„| g(sh)| “(lin)ϵ҂| ͑

xW(wng) O(sh)Ӌ(j)δ(jng)S䛱ؾ. “(lin)ϵˣX(jng)“(lin)ϵԒ13636351217 ICP䰸̖(ho)ICP11004148̖(ho)-11 L8846259

Ia(chn)ƷELISAԇСELISAԇСELISAԇСELISAԇСԇwѪM(jn)ژ(bio)(zhn)Ʒ΢B(yng)

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