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HDAC3,˽Mȥø3ELISAԇмg(sh)ָ(do)

HDAC3,˽Mȥø3ELISAԇмg(sh)ָ(do)

rh

ُȣ158    ԭa(chn)أ

l(f)rg2018/8/8 15:39:18

HDAC3,˽Mȥø3ELISAԇмg(sh)ָ(do)wⶨzyѪ塢Ѫ{M(x)弰P(gun)Һwӱ˽Mȥø3HDAC3ĺ

(bio)HDAC3   ˽Mȥø3   ELISAԇмg(sh)ָ(do)   

Ԕ(x)Ϣ
 a(chn)Ʒ
HDAC3,˽Mȥø3elisaԇg(sh)ָ(do)
z(bio)ѪѪ{ҺظˮXҺ(x)B(yng)ȡ
Ҏ(gu) 96T
Һw
棺2-8
Ҫδṩԇ
1. (bio)(zhn)Ҏ(gu)ø(bio)x
2. xęC
3. 늟B(yng)
4. ɃԇܺEppendof
5. ϵп{(dio)(ji)Һ^һΙzyƷ^röͨҺ
6. sˮƿ

HDAC3,˽Mȥø3ELISAԇмg(sh)ָ(do)E
_ʼǰԇ(yng)ƽҜ(ԇֱ37ܽ)ԇƷϡጕrrMǰ(yng)A(y)yƷƷ^ߕr(yng)ƷMϡጣʹϡጺĘƷԇеęzyӋrٳ(yng)ϡጱ(sh)
1.ӘӣքeO(sh)հ׿(bio)(zhn)סyƷհ׿׼ӘƷϡҺ100μl׷քeӘ(bio)(zhn)ƷyƷ100μlעⲻҪКӘӌƷø(bio)׵ײM|ױpp΄ӻø(bio)wĤ37淴(yng)120鱣CY(ji)ЧÿΌՈʹµĘ(bio)(zhn)ƷҺ
2.ȥҺw˦ϴÿ׼әzyҺAҺ 100μl(ʹǰһСr(ni))ø(bio)ϸĤ, 37淴(yng)60犡
3.60犺ȥ׃(ni)Һw˦ϴ3ÿν1-2s400μl/ÿ˦(ҲpČ׃(ni)Һwĸ)
4.ÿ׼әzyҺBҺ(ͬzyAҺ) 100μlø(bio)ϸĤ37淴(yng)60
5.60犺󣬗ȥ׃(ni)Һw˦ɣϴ5ÿν1-2350μl/ÿ˦(ҲpČ׃(ni)Һwĸ)
6.ÿ׼ӵҺ90μlø(bio)ϸĤ37ܹ@ɫ(30犃(ni)˕rۿҊ(bio)(zhn)Ʒǰ3-4@ݶmɫ3-4ݶȲ@ɽKֹ)
7.ÿ׼ӽKֹҺ50μlKֹ(yng)˕r{ɫD(zhun)SɫKֹҺļ(yng)McҺļͬ˱CY(ji)Ĝ(zhn)_ﷴ(yng)rg(yng)MKֹҺ
8.ø“(lin)x450nmLy׵Ĺܶ(ODֵ) ڼӽKֹҺMЙzy

ע
HDAC3,˽Mȥø3ELISAԇмg(sh)ָ(do)ע헣
1.ԇ(zhn)䣺ԇʹǰ_ҜʹúՈfҪ󱣴ԇՈʹһԵ^⽻Ⱦ
2.ӘӣӘӻԇrՈעȡ(bio) / (bio)(zhn)ƷøY(ji)rc*һ׼Ә֮gĕrgg̫(do)²ͬ “A(y)"rgĶ@Ӱ푵yֵĜ(zhn)_Լ؏(f)һμӘӕrg((bio)(zhn)ƷИƷ)10犃(ni)(bio)(sh)]ʹöҺӘӡ
3.ֹƷl(f)ԇr(yng)Н񲼵]Ѓ(ni)ø(bio)wĤԱҺwl(f)ϴ(yng)MM²κΕr(yng)ø(bio)̎ڸB(ti);ͬr(yng)(yn)ؽoķrg͜ض
4.ϴ죺ϴ^з(yng)КϴҺ(yng)ڞVϳĸVֱӷ뷴(yng)ˮͬrҪךҺwָӡӰ*ø(bio)xx(sh)
5.ԇƣDetection ADetection BʹǰՈ˦»ٕrx̎ʹܱڻƿwҺweܵ(bio)(zhn)ƷzyҺAҺzyҺBҺՈ(j)ʹãʹ(yng)ϡҺܻՈ_Ø(bio)(zhn)ƷҺMҪ΢(ȡzyҺArһβҪС10μl)Աڲ(zhn)_ϡጶɵĝ`Ո؏(f)ʹϡ^Ę(bio)(zhn)ƷzyҺAҺzyҺBҺ
6.(yng)rgĿƣՈr^췴(yng)׵ɫ׃(ÿ10)ɫ^Ո?zh)ǰKֹҺKֹ(yng)ⷴ(yng)^ĶӰø(bio)xܶx(sh)
7.ՈܹⱣ棬ڃ͜r⏊ֱhzyƷrO(sh)pלyԱCzyY(ji)Ĝ(zhn)_
(bio)дy|(zh)^ՈϡጺٜyӋrՈ*ϡጱ(sh)
˾Ҋa(chn)Ʒ
˳Ҋelisaԇϵ
ˣIL-1IL-1αIL-1βIL-2IL-2RIL-27IL-23IL-17IL-18IL-9IL-8IL-6IL-5IL-4IL-2IL-13IL-11IL-10IL-12P70IL-12P40IFN-ωIFN-βIFN-α IFN-γTNF-βTNF-αsICAM-1LEPMMP-3
ҊELISAԇϵ
TNF-αIL-1βIL-7IL-32IL-21IL-27IL-23IFN-γTGF-αTGF-β1
СҊELISAԇϵ
СIFN-γIL-1αIL-1βIL-13IL-10IL-11IL-17TNF-αTNF-βVEGFTGF-β1
iuֲȶNNELISAԇԇн(jng)^CCЧ؏(f)Լ

•rg2024/11/18 9:49:38

ھ

ھԃ𾴵Ŀ͑ã҂M؏(f)ԃ

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“(lin)ϵԒ* ߀]ݔ“(lin)ϵԒ
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xW(wng) O(sh)Ӌδ(jng)S䛱ؾ. “(lin)ϵˣX(jng)“(lin)ϵԒ13636351217 ICP䰸̖ICP11004148̖-11 L8844227

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