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ELISAԇELISAԇELISAԇСELISAԇԇwѪ塢M(jn)ژ(bio)(zhn)Ʒ΢B(yng)
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˽Mȥø1HDAC1ELISAԇf

˽Mȥø1HDAC1ELISAԇf

r(ji)h

ُ(gu)ȣ156    ԭa(chn)أ

l(f)r(sh)g2018/8/8 15:38:13

˽Mȥø1HDAC1ELISAԇfwⶨzy(c)ѪѪ{M(x)弰P(gun)Һwӱ˽Mȥø1HDAC1ĺ

Ԕ(x)Ϣ
 a(chn)Ʒ(jin)
˽Mȥø1HDAC1elisaԇf
z(bio)Ѫ塢Ѫ{ҺظˮXҺ(x)B(yng)
Ҏ(gu) 96T
Һw
棺2-8
Ҫδṩԇ
1. (bio)(zhn)Ҏ(gu)ø(bio)x
2. xęC(j)
3. 늟B(yng)
4. ɃԇܺEppendof
5. ϵп{(dio)(ji)Һ^һΙzy(c)Ʒ^r(sh)öͨҺ
6. sˮƿ

˽Mȥø1HDAC1ELISAԇfE
(sh)(yn)_ʼǰԇ(yng)ƽҜ(ԇֱ37ܽ)ԇƷϡጕr(sh)r(sh)M(sh)(yn)ǰ(yng)A(y)y(c)ƷƷ^ߕr(sh)(yng)(du)ƷM(jn)ϡʹϡጺĘƷԇеęzy(c)Ӌ(j)r(sh)ٳ(yng)ϡጱ(sh)
1.ӘӣքeO(sh)հ׿(bio)(zhn)y(c)Ʒհ׿׼ӘƷϡҺ100μl׷քeӘ(bio)(zhn)Ʒy(c)Ʒ100μlעⲻҪКӘӌƷø(bio)׵ײM|ױpp΄(dng)ø(bio)wĤ37淴(yng)120鱣C(sh)(yn)Y(ji)ЧÿΌ(sh)(yn)Ո(qng)ʹµĘ(bio)(zhn)ƷҺ
2.ȥҺw˦ϴÿ׼әzy(c)ҺAҺ 100μl(ʹǰһСr(sh)(ni))ø(bio)ϸĤ, 37淴(yng)60
3.60犺󣬗ȥ׃(ni)Һw˦ɣϴ3ÿν1-2犣s400μl/ÿ˦(ҲpČ׃(ni)Һwĸ)
4.ÿ׼әzy(c)ҺBҺ(ͬzy(c)AҺ) 100μlø(bio)ϸĤ37淴(yng)60
5.60犺ȥ׃(ni)Һw˦ɣϴ5ÿν1-2350μl/ÿ˦(ҲpČ׃(ni)Һwĸ)
6.ÿ׼ӵҺ90μlø(bio)ϸĤ37ܹ@ɫ(30犃(ni)˕r(sh)ۿҊ(bio)(zhn)Ʒǰ3-4@ݶmɫ3-4ݶȲ@ɽKֹ)
7.ÿ׼ӽKֹҺ50μlKֹ(yng)˕r(sh){(ln)ɫD(zhun)SɫKֹҺļ(yng)McҺļͬ˱C(sh)(yn)Y(ji)Ĝ(zhn)_ﷴ(yng)r(sh)g(yng)MKֹҺ
8.ø“(lin)x450nmL(zhng)y(c)׵Ĺܶ(ODֵ) ڼӽKֹҺM(jn)Йzy(c)

ע(xing)
˽Mȥø1HDAC1ELISAԇf(sh)(yn)ע(xing)
1.ԇ(zhn)䣺ԇʹǰ_(d)ҜʹúՈ(qng)fҪ󱣴ԇ(sh)(yn)Ո(qng)ʹһԵ^⽻Ⱦ
2.ӘӣӘӻԇr(sh)Ո(qng)עȡ(bio) / (bio)(zhn)ƷøY(ji)r(sh)(g)c*һ(g)׼Ә֮gĕr(sh)gg̫(hu)(do)²ͬ “A(y)"r(sh)gĶ@Ӱ푵y(c)ֵĜ(zhn)_Լ؏(f)һμӘӕr(sh)g((bio)(zhn)ƷИƷ)10犃(ni)(bio)(sh)]ʹöҺӘӡ
3.ֹƷl(f)ԇ(yn)r(sh)(yng)Н񲼵]Ѓ(ni)ø(bio)wĤԱҺwl(f)ϴ(yng)MM(jn)²κΕr(sh)(yng)ø(bio)̎ڸB(ti);ͬr(sh)(yng)(yn)ؽoķr(sh)g͜ضȡ
4.ϴ죺ϴ^з(yng)КϴҺ(yng)ڞVϳĸVֱӷ뷴(yng)ˮͬr(sh)ҪךҺwָӡӰ*ø(bio)xx(sh)
5.ԇƣDetection ADetection BʹǰՈ(qng)˦»ٕr(sh)x̎ʹܱڻƿwҺweܵ(bio)(zhn)Ʒzy(c)ҺAҺzy(c)ҺBҺՈ(qng)(j)ʹãʹ(yng)ϡҺܻՈ(qng)_Ø(bio)(zhn)ƷҺMҪ΢(ȡzy(c)ҺAr(sh)һβҪС10μl)Աڲ(zhn)_ϡጶɵĝ`Ո(qng)؏(f)ʹϡ^Ę(bio)(zhn)Ʒzy(c)ҺAҺzy(c)ҺBҺ
6.(yng)r(sh)gĿƣՈ(qng)r(sh)^췴(yng)׵ɫ׃(ÿ10)ɫ^Ո(qng)?zh)ǰKֹҺKֹ(yng)ⷴ(yng)^(qing)ĶӰø(bio)xܶx(sh)
7.Ո(qng)ܹⱣڃ(ch)͜r(sh)⏊(qing)ֱhzy(c)Ʒr(sh)O(sh)pלy(c)ԱCzy(c)Y(ji)Ĝ(zhn)_
(bio)дy(c)|(zh)^Ո(qng)ϡጺٜy(c)Ӌ(j)r(sh)Ո(qng)*ϡጱ(sh)
˾Ҋa(chn)Ʒ
˳Ҋelisaԇϵ
ˣIL-1IL-1αIL-1βIL-2IL-2RIL-27IL-23IL-17IL-18IL-9IL-8IL-6IL-5IL-4IL-2IL-13IL-11IL-10IL-12P70IL-12P40IFN-ωIFN-βIFN-α IFN-γTNF-βTNF-αsICAM-1LEPMMP-3
ҊELISAԇϵ
TNF-αIL-1βIL-7IL-32IL-21IL-27IL-23IFN-γTGF-αTGF-β1
СҊELISAԇϵ
СIFN-γIL-1αIL-1βIL-13IL-10IL-11IL-17TNF-αTNF-βVEGFTGF-β1
iuֲȶNNELISAԇԇн(jng)^(yn)CCЧ؏(f)Լ

•r(sh)g2024/11/18 9:49:38

ھ

ھԃ𾴵Ŀ͑҂(hu)M؏(f)ԃ

dȤĮa(chn)Ʒ* ߀]ݔdȤĮa(chn)Ʒ
Ćλ* ߀]ݔĆλ
“(lin)ϵ* ߀]ݔ“(lin)ϵ
“(lin)ϵԒ* ߀]ݔ“(lin)ϵԒ
Ԕ(x)ַ
] ݔ]ʽ_
Ո(qng)ݔ(du)҂Ҋh* ߀]ݔ(du)҂Ҋh
(yn)Ca* ߀]ݔ(yn)Ca

l(f)

ܰʾ

1.A񹲺͇(gu)P(gun)Ҏ(gu)ؾW(wng)ϵГ(dn)һОֱӻgķ؟(z)Ρ

2.Ո(qng)挍(sh)ķӳa(chn)Ʒr,Ҫ_{猦(du)a(chn)ƷκɆ,Ҳԃ

3.δ(jng)վͬκ˲ñԲl(f)(g)˻F(tun)wľЏV|(zh)ϢՓ

P(gun)

| ˾B| a(chn)Ʒչʾ| ˾„| g(sh)| “(lin)ϵ҂| ͑

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