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(dng)ǰλãϺh(yun)\g(sh)l(f)չ˾ > a(chn)Ʒ > ELISAԇ > ELISAԇЃr(ji) > ˿涡CotinineELISAԇЃr(ji)
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l(f)r(sh)g2018/5/3 14:25:29
a(chn)ƷQ˿涡Cotinineelisaԇr(ji)
λУ/t(y)Ժ/Ժ
(sh)(yn)ͣAķ/gӷ/
zy0.75 pg/mL – 24 pg/mL
(yng)r(sh)g: 1-5h
ӱwe: 50-100ul
zyL: 450 nm
`ȣzyС0.1 pg/mL
خԣcԽY(ji)(gu)サ淴(yng)
؏(f)ԣ(ni)׃ϵ(sh)С10% g׃ϵ(sh)С15%
(yng)÷wⶨzyѪѪ{M(x)弰P(gun)Һwӱ
l2-8棻
a(chn)ƷHЌ(sh)(yn)mR\
a(chn)Ʒ
יzyϵУԇ,TNFԇ,VEGFԇ,IpԇУ
P(gun)СәzyϵУƤ|(zh)ԇ,HCYԇ,T3,T4ԇ
ʳƷȫСәzyϵУcùELISAzyԇ,谷ELISAzyԇ,ĭh(hun)ELISAzyԇ,SùELISAzyԇУ
wzyϵУѪowELISAzyԇ,AQP-Abԇ,HINIԇ
طNיzyʮ(g)Nٵİ,ELISAzyԇ
M(fi)y
ֻјӱ^҂?yu)?ji)ʡr(sh)gY(ji)ԭʼ(sh)(j)(sh)(j)ṩr(sh)g5-7ң
g(sh)ָ(do)
ȫ̼g(sh)ָ(do)ǰĘ(bio)ռʹ^вĵط(sh)(yn)Y(ji)Ĕ(sh)(j)ȵȣ߅elisaԇЌ(sh)(yn)
˿CotinineELISAԇЃr(ji)͑Ⱥw
ӆُ̣
gӭĿІλڸN(xing)Ŀc˾_չͬӴεкpAl(f)չͬM(jn)ЇzyI(y)İl(f)չe۽(jng)(yn)
E
1. Ҝƽ20minXȡlʣlԷܷŻ4档
2. O(sh)Ø(bio)(zhn)Ʒ͘ӱ(bio)(zhn)ƷӲͬȵĘ(bio)(zhn)Ʒ50μL
3. ӱмyӱ50μLհײ
4. հ⣬(bio)(zhn)Ʒ͘ӱÿ^øHRP(bio)ӛęzyw100μL÷Ĥס(yng)ף37ˮԡ偻60min
5. ȥҺwˮĸÿӝMϴҺ350μLo1min˦ȥϴҺˮĸ؏(f)ϴ5ΣҲϴC(j)ϴ壩
6. ÿAB50μL37ܹ15min
7. ÿKֹҺ50μL15min(ni)450nmL̎yODֵ
˿涡CotinineELISAԇЃr(ji)ע(xing)
1.(yn)Ҏ(gu)ĕr(sh)g͜ضM(jn)МԱC(zhn)_Y(ji)ԇʹǰ_(d)Ҝ20-25档ʹúرԇ
2.ϴ岻_Ԍ(do)²(zhn)_ĽY(ji)ڼǰ_Mɿ׃(ni)Һw^вҪ
3.ךҺwָӡtӰODֵ
4.@ɫҺ(yng)ʟoɫܜ\ɫѽ(jng)׃{(ln)ĵҺʹá
5.ԇ͘(bio)ĽȾe(cu)`Y(ji)
6.ڃ(ch)͜r(sh)⏊(qing)ֱ
7.ƽҜغٴ_ܷԷˮlϡ
8.κη(yng)ԇܽ|Ư܄Ư܄ɢl(f)ď(qing)ҚwκƯ׳ɷֶ(hu)Ɖԇз(yng)ԇ
9.ʹ^ڮa(chn)Ʒ
10.܂еĘƷ(yng)Ҏ(gu)ij̎Ʒ͙zyb
r(sh)g2024/11/18 9:49:20