【文獻標題】Programming changes in GLUT1 mediated the accumulation of AGEs ａnd matrix degradation in the articular cartilage of female adult rats after prenatal caffeine exposure

【作者】Qing-xian Li，Lin-long Wang，et.al

【作者單位】武漢大學中南醫(yī)院（Zhongnan Hospital of Wuhan University）

【文獻中引用產(chǎn)品】

試劑-藏紅o試劑(CAS. No. 477-73-6)

試劑-固綠FCF試劑(CAS. No. 2353-45-9)

【關(guān)鍵詞】Prenatal caffeine exposure，Glucocorticoid，Glucose transporter 1，Glucose metabolism，Articular cartilage，Cartilage development

【DOI】https://doi.org/10.1016/j.phrs.2019.104555

【影響因子(IF)】9.90

【出版期刊】《Pharmacological Research》

【產(chǎn)品原文引用】

Caffeine (CAS #58-08-2, > 99% purity) was purchased from SigmaAldrich Co., Ltd. (St. Louis, MO, USA). Isoflurane was purchased from Baxter Healthcare Co. (Deerfield, IL, USA). Safranin O (CAS No. 477-73-6) and Fast Green (CAS No. 2353-45-9) were obtained from Hengyuan Biotech (Shanghai, China). A TRIzol reagent (SKU: R6830-02) was purchased from Omega Bio-Tek (Doraville, GA, USA). Reverse transcription ａnd quantitative PCR kits were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Oligonucleotide primers for the gene used for analysis were synthesized by Sangon Biotech Co., Ltd.(Shanghai, China). RIPA lysis buffer ａnd a BCA Protein Assay kit were purchased from Beyotime Institute of Biotechnology (Haimen, China).Western Bright ECL HRP substrate was purchased from Advansta (Menlo Park, CA, USA). Fetal bovine serums were supplied by Gibco (St.Louis, MO, USA). Recombinant Murine IGF1 (250-19) was purchased from PeproTech, Inc. (Rocky Hill, NJ, USA). Advanced glycation end products (AGE-BSA, 2221-BSA) ａnd BSA (2221-10) were purchased from BioVision (Milpitas, CA, USA). Low molecular weight heparin (LMWH) was purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO,USA). Collagenase II was obtained from Invitrogen (Carlsbad, CA, USA).Polyclonal antibody information is as follows: glyceraldehyde 3-phosphatedehydrogenase (GAPDH) (No. AC033), anti-β-actin (No.AB_2768234), α1 chain of type II collagen gene (COL2A1) (No.ABP51023), aggrecan (ACAN) (No. bs-1223R), GLUT1 (No. 21829-1-AP), AGEs (No. bs-1158R), ADAM metallopeptidase with thrombospondin type 1 motif 5 (ADAMTS-5) (No. ab41037), matrix metallopeptidase 13 (MMP13) (No. ab39012), IGF1 (No. sc-9013), ａnd acetylhistone 3 lysine 9 (H3K9) (No. A7255). Secondary antibody information is as follows: Cy3 conjugated goat anti-rabbit IgG (H + L) (No.B100801) ａnd Cy3 conjugated goat anti-rabbit IgG (H + L) (No. AS-1111). The other chemicals ａnd agents were analytical grade.

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